Zakariyya, H.Y., Abdullahi, M. N. and Fatima, M.M.

Department of Science Laboratory Technology, Hussaini Adamu Federal Polytechnic, Kazaure, Jigawa State. Nigeria

Abstract:

The methanolic extracts of the leaves and stem bark of Anogeissus leiocarpus were investigated for thier Phytochemical compounds and in-vitro antimicrobial activity against clinical isolates of Pseudomonas aeruginosa, Salmonella typhii and Escherichia coli using agar diffusion techniques. The result of the Phytochemical screening of the plant parts revealed the presence of carbohydrates, flavonoids, tannins, saponins, glycosides, steroids, anthraquinones and glycosides.  While the result of the anti-microbial assay showed that the extracts of the leaves and stem bark had significantly inhibited the growth of the tested micro-organisms which increased with increase in the concentration of the extracts (25, 50, 100mg/ml). However, the leaves extract inhibited much better that the extract of the stem bark most especially as it shielded Pseudomonas- aeruginosa(0.6,0.6 and 0.8 mm) then Salmonella typhii (0.6,0.6 and 0.4mm)and lastly Escherichia coli(0.6,0.5 and 0.0mm). Some of these bioactive components found in the plant may be responsible for its anti-microbial activities as observed in other medicinal plants. In the same vain this study justified the use of the plant in wound healing and similar ailments amongst the people growing it in their locality.

Keywords: Anogeissus leiocarpus, clinical isolates, antimicrobial activity and bioactive components.